| 中国沿海尖刺拟菱形藻的种下分类学研究* |
| |
| 引用本文： | 董焕嫦,黄春秀,徐国双,李扬. 中国沿海尖刺拟菱形藻的种下分类学研究*[J]. 热带海洋学报, 2018, 37(1): 12-19. DOI: 10.11978/2017017 |
| |
| 作者姓名： | 董焕嫦 黄春秀 徐国双 李扬 |
| |
| 作者单位： | 1. 华南师范大学生命科学学院, 广州市亚热带生物多样性与环境生物监测重点实验室, 广州 广东 5106312. 华南师范大学生命科学学院, 广东省水产健康安全养殖重点实验室, 广州 广东 510631 |
| |
| 基金项目： | 国家自然科学基金(31570205、31370235);广州市科技计划项目(201607010370) |
| |
| 摘 要： | 为了澄清中国沿海尖刺拟菱形藻Pseudo-nitzschia pungens Hasle的种下分类学现状, 从我国沿海分离并建立了32个尖刺拟菱形藻的单克隆培养株系, 结合光学显微镜下的群体特征和透射电镜下的超微形态学特征, 以及基于核糖体转录间隔区(ITS)的分子系统学数据, 鉴定得到尖刺拟菱形藻的两个变种: 尖刺拟菱形藻原变种P. pungens var. pungens和尖刺拟菱形藻阿维罗变种P. pungens var. averiensis。文章对其形态学特征进行了详细描述, 并利用高效液相色谱-质谱联用法(LC-MS/MS)对培养株系的多莫酸(DA)进行了检测, 但未检测到DA。
|
| 关 键 词： | 尖刺拟菱形藻 种下分类学 形态 核糖体转录间隔区 多莫酸 |
| 收稿时间： | 2017-02-15 |
| 修稿时间： | 2017-05-26 |
Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters* |
| |
| Huanchang DONG,Chunxiu HUANG,Guoshuang XU,Yang LI. Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters*[J]. Journal of Tropical Oceanography, 2018, 37(1): 12-19. DOI: 10.11978/2017017 |
| |
| Authors: | Huanchang DONG Chunxiu HUANG Guoshuang XU Yang LI |
| |
| Affiliation: | 1. Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, College of Life Science, South China Normal University, Guangzhou 510631, China2. Guangdong Provincial Key Laboratory of Healthy and Safe Aquaculture, College of Life Science, South China Normal University,Guangzhou 510631, China |
| |
| Abstract: | To declare intraspecies diversity of Pseudo-nitzschia pungens Hasle, a total of 32 monoclonal strains of P. pungens from Chinese coastal waters were isolated and established. These strains were identified carefully, based on the morphology under both light microscopy and transmission electron microscopy, and on the molecular analysis inferred from the internal transcribed spacer region sequences. Two varieties were identified: P. pungens var. pungens (Grunow) Hasle and P. pungens var. averiensis Lundholm, Churro, Carreira, and Calado. Their morphologies were described and compared with allied taxa. Meanwhile, domoic acid (DA) was detected using a liquid chromatography tandem mass spectrometry (LC-MS/MS) injection, but no detectable DA was found. The results may provide detailed information for further studies of toxin production of Chinese Pseudo-nitzschia strains. |
| |
| Keywords: | Pseudo-nitzschia pungens taxonomy morphology internal transcribed spacer domoic acid |
|
| 点击此处可从《热带海洋学报》浏览原始摘要信息 |
|
点击此处可从《热带海洋学报》下载全文 |
|
