盐度胁迫对小黄鱼(Larimichthys polyactis)抗氧化酶、非特异性免疫酶和Na+/K+-ATP酶活力的影响

为了研究不同盐度对小黄鱼生理的影响,以人工养殖的4月龄小黄鱼(体质量为(12.6±3.1)g)为实验对象,将在自然海水(对照组盐度为22.1)中养殖的小黄鱼转入到盐度为5(低盐组)和34.5(高盐组)的海水中进行急性盐度胁迫处理10 d,测定并分析肝脏中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、碱性磷酸酶(AKP...

Effect of salinity stress on the antioxidant enzymes,non-specific immune enzymes,and Na+/K+-ATPase activities in Larimichthys polyactis

In order to study the physiological effects of different salinity stress on Larimichthys polyactis,the fourmonth-old L.polyactis(body weight(12.6±3.1)g)cultured in natural seawater with salinity of 22.1 were applied to salinity stress in seawater salinity of 5(called as low-salinity group)and 34.5(called as high-salinity group),and natural seawater was used as a control group.After 10 days of acute salinity stress experimental treatment,the activities of superoxide dismutase(SOD),catalase(CAT),alkaline phosphatase(AKP)and acid phosphatase(ACP)in the liver and Na⁺/K⁺-ATPase in gills and kidneys were measured and analyzed.The results showed that under acute salinity stress,the activity of antioxidant enzymes(SOD and CAT)in the liver were increased.Among them,the activities of SOD in the low-salinity group and CAT in the high-salinity group were significantly higher than those in the control group(p<0.05).Under different salinity conditions,AKP and ACP showed an opposite trend of change,i.e.AKP activity gradually increased with increasing salinity,while ACP activity gradually decreased.Additionally,Na⁺/K⁺-ATPase activity in gills was the lowest in the low salinity group,and the activity of which in the kidney in the high salinity group was significantly higher than that of the control group(p<0.05).The above research results indicate that juveniles of L.polyactis can still survive normally when the salinity drops to 5,without obvious abnormality.And different salinity stress can significantly affect the non-specific immune enzymes activities of the liver,and the Na⁺/K⁺-ATPase activity of the gills and kidneys in L.polyactis.In other word,the tissues of liver,gill and kidney play an important role during the process of L.polyactis adapting to changes in salinity.The research results also provide a reference for the cultivation of L.polyactis in high-saline or brackish water areas.