红壤中微生物群落对半导体矿物日光催化作用的响应

本研究选取天然红壤作为研究对象，分析其中的含铁半导体矿物光催化作用对本源微生物群落结构的影响。采用聚合酶链式反应/变性梯度凝胶电泳（PCR-DGGE），研究了不同原始光照条件的红壤中微生物群落在外源电子作用下群落结构的改变。DGGE图谱的主成分分析表明，两个不同原始光照环境的土壤样品的微生物群落结构由于原始环境的差异而不同：原始环境为强光照的样品中微生物群落结构受外源电子影响较小；原始环境为弱光照的样品中微生物群落结构受外源电子影响较大。这一群落结构改变的差异可能由于原始环境为强光照时半导体矿物光催化作用产生光生电子传递到环境中持续影响周围的微生物群落，而原始弱光照环境中则缺少光生电子的作用。

Response of Microbial Community to Sunlight Catalysis of Semiconductor Minerals in Red Soil

The natural  red soil was used  in  this study as a  research object, and  the  influence of semiconductor photocatalysis of  iron-bearing minerals on microbial community was analyzed. Using  the polymerase chain  reaction/degeneration gradient gel electrophoresis  (PCR-DGGE),  the microbial community  from  the original  red soil samples under different  light conditions was investigated after being disposed with exogenous electrons supplement. Principal component analysis  (PCA) of DGGE banding patterns indicated that the difference of the microbial community structure in two soil samples was due to different original light environments.  In  the  red soil with strong  light, no significant change of  the microorganism community structure was observed by processing with exogenous electrons; while in the original environment with weak light, the structure of microbial community was significantly affected by  the exogenous electrons. The different response of microbial community structure  to  the exogenous electrons may be attributed to the photoinduced electrons generated by the semiconducting mineral photocatalysis in the primitive strong  irradiation environment, which continued  to affect  the surrounding microbial communities; while photoinduced electrons were inadequate in the weak irradiation environment.