Detection of coenzyme F430 in deep sea sediments: A key molecule for biological methanogenesis |
| |
| Institution: | 1. Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka 237-0061, Japan;2. Submarine Resources Research Project, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka 237-0061, Japan;3. Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse 10, D-35043 Marburg, Germany;4. PRESTO, Japan Science and Technology Agency (JST), Honcho, Kawaguchi, Saitama 332-0012, Japan |
| |
| Abstract: | We report the presence of coenzyme factor 430 (F430), a prosthetic group of methyl coenzyme M reductase for archaeal methanogenesis, in the deep sub-seafloor biosphere. At 106.7 m depth in sediment collected off Shimokita Peninsula, northwestern Pacific, its concentration was estimated to be at least 40 fmol g sediment?1 (i.e. 36 pg g?1 wet sediment). This is about three orders of magnitude lower than typical concentrations of archaeal intact polar lipids in similar sub-seafloor sediments. On the basis of the concentration of F430 in methanogens and conversion to biomass composed of typical sub-seafloor microbial cells, we estimated that ca. 2 × 106 cells g?1 could be methanogens in the deeply buried marine sediment. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
