患病与健康杂色鲍苗中弧菌胞外产物的比较分析

本实验室于2004年采样自广东深圳下沙鲍鱼场变白掉板杂色鲍苗以及官湖养殖场健康鲍苗,各分离到16和34株弧菌,并对其胞外产物进行了比较分析。结果表明,官湖场健康鲍苗弧菌与下沙场患病鲍苗弧菌相比,前者20.59%(7株)菌株虽不分泌胞外酶但具溶血活性,余之菌株(64.71%,22株)或分泌酶和/或具溶血活性,但同时兼具蛋白酶、明胶酶、卵磷脂酶、脂肪酶和溶血活性的极少(5.88%,2株);后者则所有菌株均分泌胞外酶和/或具溶血活性,25.00%(4株)菌株能分泌四种酶及具溶血毒性,其余菌株(68.75%,11株)可分泌多种酶和/或具溶血活性。结果揭示,下沙鲍鱼场患病鲍苗弧菌分泌胞外酶和具溶血活性的能力明显强于官湖场健康鲍苗弧菌的,同时认为菌株X99-2和X100-8与下沙鲍苗病害潜在相关。

COMPARISONS OF VIRULENCE OF VIBRIONS ISOLATED FROM BOTH DISEASED AND HEALTHY POSTLARVAE OF ABALONE

Since 2002,massive death of abalone postlarvae has swept across the south coast of China.To explore the cause behind it,an investigation has been carried out to compare the abilities of vibrios to produce extracellular virulent factors from different settings.In this study,16 and 34 vibrios were isolated from both diseased and healthy postlarvae of abalone in 2004,respectively.Comparisons of their extracellular virulent factor producing abilities revealed that 20.59%of vibrios from the healthy postlarvae did not produce four extracellular enzymes but could lyse goat blood agar,64.71% produced enzymes or hemolysis.The rest possessed weak abilities to produce four enzymes and/or hemolysis.Contrarily,100% vibrios from diseased postlarvae produced extracellular enzymes or hemolysis.Among them,25% produced four enzymes and hemolysis,68.75% produced either extracellular enzymes or haemolysis on goat blood agar.In conclusion,vibrios from diseased postlarvae showed better abilities to produce extracellular protease,gelatinase,phospholipase and lipase as well as hemolysis than those from the healthy postlarvae.Furthermore,strains x99-2 and x100-8 were considered to be potential pathogens on the basis of their abilities to produce extracellular virulent factors and warrant further studies.