HPLC determination of phytoplankton pigments using N,N-dimethylformamide |
| |
| Authors: | Ken Furuya Masato Hayashi Yasushi Yabushita |
| |
| Affiliation: | (1) Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, 113-8657 Tokyo, Japan;(2) Faculty of Bioresources, Mié University, Kamihama, Tsu, 514-0008 Mié, Japan;(3) Present address: Akagi Suisan Co., 3211, Handa, Shibukawa, 377 Gunma, Japan |
| |
| Abstract: | The suitability of N,N-dimethylformamide (DMF) as an extractant for the standard reverse-phase HPLC method was examined using algal cultures. Good pigment separations and recovery were achieved with 20% (volume %) addition of an ion-pairing solution in an injection. While slight amounts of degradation products of chlorophylla, i.e., chlorophyllidea, allomeric and epimeric forms, were produced, adequate attention to filtration and extraction prevents the formation of degradation products, confining them to an acceptable level. Because of its strong extractability, which expedites the extraction process, DMF is an efficient solvent for HPLC analysis of phytoplankton pigments. |
| |
| Keywords: | Pigments HPLC N,N-dimenthylformamide phytoplankton chlorophylla |
| 本文献已被 SpringerLink 等数据库收录！ |
|
