| 6—DMAP抑制九孔鲍受精卵第二极体的释放诱导三倍体 |
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| 引用本文： | 严正凛,吴萍茹,高霞灵,陈建华. 6—DMAP抑制九孔鲍受精卵第二极体的释放诱导三倍体[J]. 台湾海峡, 2001, 20(1): 15-19 |
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| 作者姓名： | 严正凛 吴萍茹 高霞灵 陈建华 |
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| 作者单位： | 1. 福建海洋研究所,
2. 福建省漳浦县水产局, |
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| 基金项目： | 福建省科学技术项目(96-Z-171) |
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| 摘 要： | 当50％九孔鲍受精卵出现第一极体后，用6－DMAP不同的浓度进行不同的持续时间诱导三倍体。结果表明，6－DMAP的诱导浓度为300μmol/dm^3、诱导持续时间为10min时，其诱导的三倍体率为90％以上，并且胚胎的孵化率高达85％，幼体的畸形率较低，为50％－55％。用该方法多次进行了九孔鲍三倍体生产诱导和苗种培育试验。本文还对6－DMAP的作用机理以及九孔鲍三倍体诱导中的诱导因子等问题进行了讨论。
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| 关 键 词： | 九孔鲍 6－二甲基氨嘌呤 三倍体 诱导 受精卵 第二极体 作用机理 |
| 文章编号： | 1000-8160（2001）01-0015-05 |
Triploid of the abaloneHaliotis diversicolor supertexta induced with 6-DMAP:by blocking the second polar body ofthe zygotes |
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| YAN Zheng-lin,WU Ping-ru,GAO Xia-ling,CHEN-Jianhua. Triploid of the abaloneHaliotis diversicolor supertexta induced with 6-DMAP:by blocking the second polar body ofthe zygotes[J]. Journal of Oceanography In Taiwan Strait, 2001, 20(1): 15-19 |
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| Authors: | YAN Zheng-lin WU Ping-ru GAO Xia-ling CHEN-Jianhua |
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| Abstract: | When 50% of zygotes of Haliotis diversicolor supertexta develop polar body I,6-DMAP at various concentration are used to induce triploid at different treatmenttime.Results show that when 6-DMAP is at concentration of 300μmol/dm3,and treatment time is 10 min,the rate of inducing triploid can reach above 90%.In addition,embryonic hatching rate is at 85%,with larval deformity rate around 50%～55%. Many tests have been conducted under this method to induce triploid of Haliotis diversicolor aquatilis and larvae rearing experiment.This article also covers discussi on sonfunctional mechanism of 6-DMAP and factors of inducing triploid from Haliotis diversicolor supertexta. |
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| Keywords: | Haliotis diversicolor supertexta 6 Dimethylaminopurine(6 DMAP) triploid induction |
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