| REPRODUCTIVE MECHANISM OF RUDITAPES PHILIPPINARUM ISOLATION OF SHEDDING HORMONE AND DETERMINATION OF BIOLOGICAL ACTIVITY |
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| 作者姓名： | 方永强 张玉生 许瑞安 张其森 |
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| 作者单位： | Third Institute of Oceanography，National Bureau of Oceanography，Xiamen，Third Institute of Oceanography，National Bureau of Oceanography，Xiamen，Third Institute of Oceanography，National Bureau of Oceanography，Xiamen，Third Institute of Oceanography，National Bureau of Oceanography，Xiamen |
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| 摘 要： | An active substance, shedding hormone capable of inducing parent clams to spawn and sper-miate, has preliminarily been isolated from the ovaries of Ruditapes philippinarum by means of ammonia sulfate precipitate of 25% concentration and Sephadex G-25 column chromatography in present study. The biological activity of the shedding hormone is above 95%. It is composed of sorts of amino acids by determination with an amino acid automatic analyzer. The experimental results indicate that the active substance is a polypeptide.
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| 收稿时间： | 1982/11/29 0:00:00 |
| 修稿时间： | 1984/1/19 0:00:00 |
REPRODUCTIVE MECHANISM OF RUDITAPES PHILIPPINARUM ISOLATION OF SHEDDING HORMONE AND DETERMINATION OF BIOLOGICAL ACTIVITY |
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| FANG YONGQIANG,ZHANG YUSHENG,XU RUIAN and ZHANG QISEN.REPRODUCTIVE MECHANISM OF RUDITAPES PHILIPPINARUM ISOLATION OF SHEDDING HORMONE AND DETERMINATION OF BIOLOGICAL ACTIVITY[J].Acta Oceanologica Sinica,1985,4(1):107-112. |
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| Authors: | FANG YONGQIANG ZHANG YUSHENG XU RUIAN and ZHANG QISEN |
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| Institution: | Third Institute of Oceanography, National Bureau of Oceanography, Xiamen |
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| Abstract: | An active substance, shedding hormone capable of inducing parent clams to spawn and sper-miate, has preliminarily been isolated from the ovaries of Ruditapes philippinarum by means of ammonia sulfate precipitate of 25% concentration and Sephadex G-25 column chromatography in present study. The biological activity of the shedding hormone is above 95%. It is composed of sorts of amino acids by determination with an amino acid automatic analyzer. The experimental results indicate that the active substance is a polypeptide. |
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