饲料添加多维对花鲈生长、非特异性免疫指标及消化酶活性的影响

本试验旨在研究饲料中不同水平的多维含量对花鲈（Lateolabrax japonicus）生长性能、非特异性免疫指标及消化酶活性的影响。以初始体质量为（38.54±0.05） g的花鲈为试验对象，随机分为6组（每组3个重复，每个重复30尾鱼），基础饲料中分别添加不同水平（0.5%、1%、2%、3%、4%、5%）的多维，在室内循环养殖系统中饲喂28 d。结果表明:多维添加量对花鲈的体成分、存活率、肝指数、饲料系数和肥满度均没有显著影响（P>0.05）；当多维添加量为2%时，花鲈的增重率（WGR）和特定生长率（SGR）均最大，与多维添加量为2%相比较，随着多维添加量继续增多，花鲈的WGR和SGR显著降低（P<0.05）；当多维添加量为5%时，花鲈的肠蛋白酶活性较0.5%和1%添加量组显著提高（P<0.05），而多维添加量为0.5%时，花鲈的肠淀粉酶活性显著高于其余任意一组（P<0.05），各组之间肠脂肪酶活性无显著差异（P>0.05）；与多维添加量为0.5%相比，多维添加量为5%时，能够显著提高花鲈血清中超氧化物歧化酶、碱性磷酸酶活性以及血清中总蛋白含量（P<0.05），而血清溶菌酶活性没有显著变化（P>0.05）。结果表明:饲料中添加多维含量为5%时，能够明显提高花鲈的非特异性免疫指标及肠道蛋白酶活性，而在含量为2%时，能够明显提高花鲈的生长性能。

Effects of multi-vitamins on the growth performance, non-specific immunity indices and digestive enzymatic activity of Lateolabrax japonicus

This report aimed to investigate the effects of basal diets by adding different dosages(0.5%, 1%, 2%, 3%, 4%, 5%) of multi-vitamins on the growth performance, non-specific immunity indices and digestive enzymatic activity of Lateolabrax japonicus. The fishes with initial body mass of (38.54±0.05)g were fed 28 days in indoor close-recirculating culture systems, and divided into six groups, each group contained 30 individuals and set in triplicate. The results showed that the dosage of multi-vitamins had no significant influence to the body composition, survival rate, hepatosmatic index and feed conversion ratio (P>0.05) of L. japonicus; the weight gain rate (WGR) and specific growth rate (SGR) reached highest at 2% diet; with the increase of multi-vitamin dosages, WGR and SGR decreased significantly (P<0.05) compared to 2% diet. The 5% diet improved the intestinal protease activity, while the intestine amylase (AMS) activity of the control group was significantly higher than any other groups (P<0.05), but there was no significant influences on intestinal LPS activity (P>0.05). Intestinal total protein (TP) content reached maximum at 3% diet with significant difference (P<0.05). The 5% diet improved the activity of total superoxide dismutase (T-SOD), alkaline phosphatase (AKP) and TP content in serum significantly (P<0.05), while serum LSZ activity had no significant change (P<0.05). In conclusion, the feed by adding 5% multi-vitamins significantly improved the non-specific immunity indices of L. japonicus and the intestinal protease activity, while the feed supplemented with 2% multi-vitamins obviously promoted the growth of L. japonicus.