| 香鱼Apo-14基因的克隆、序列分析及其mRNA表达与盐度适应性的关系 |
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| 引用本文： | 曹霄,陈炯,史雨红,陆新江.香鱼Apo-14基因的克隆、序列分析及其mRNA表达与盐度适应性的关系[J].台湾海峡,2011,30(2):203-209. |
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| 作者姓名： | 曹霄 陈炯 史雨红 陆新江 |
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| 作者单位： | 宁波大学应用海洋生物技术教育部重点实验室,浙江,宁波,315211 |
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| 基金项目： | 国家973计划前期专项资助项目，宁波市科技局资助项目，宁波大学研究生科研创新基金 |
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| 摘 要： | 14 kDa载脂蛋白（Apo-14）是鱼类一种特有的载脂蛋白,是鱼类高密度脂蛋白（HDLs）的重要成分,其生物学功能目前还不明确.本研究采用简并引物,从香鱼（Plecoglossus altivelis）肝脏cDNA文库中筛选香鱼Apo-14基因,该基因cDNA序列全长905个核苷酸,开放阅读框编码一个有144个氨基酸,分子量约为15.9 kDa的蛋白.香鱼Apo-14基因mRNA主要在香鱼肝脏中表达,在肠、肾和鳃中也有少量表达.同源性分析揭示,香鱼Apo-14与已知鱼类中虹鳟（Oncorhynchus mykiss）Apo-14最相似,氨基酸序列同源性为70%.qRT-PCR表明,咸淡水处理组（盐度为10）的肝、肠、肾、鳃中,该基因mRNA均比淡水处理组（盐度为0）明显下调（p〈0.05）,揭示载脂蛋白Apo-14可能与盐度适应相关.
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| 关 键 词： | 海洋生物学 Apo-14 克隆 序列分析 盐度适应 qRT-PCR |
Gene cloning and sequencing of Apo-14 gene in ayu (Plecoglossus altivelis) and the effects of salinity change on its mRNA expression |
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| CAO Xiao,CHEN Jiong,SHI Yu-hong,LU Xin-jiang.Gene cloning and sequencing of Apo-14 gene in ayu (Plecoglossus altivelis) and the effects of salinity change on its mRNA expression[J].Journal of Oceanography In Taiwan Strait,2011,30(2):203-209. |
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| Authors: | CAO Xiao CHEN Jiong SHI Yu-hong LU Xin-jiang |
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| Institution: | CAO Xiao,CHEN Jiong,SHI Yu-hong,LU Xin-jiang(Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo 315211,China) |
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| Abstract: | Apolipoproteins are important structural components of plasma lipoproteins and have been shown to participate in lipoprotein assembly,secretion,processing,and catabolism.14 kDa apolipoprotein(Apo-14),a specific apolipoprotein in fish,is an important component of high-density lipoproteins,but its biological functions remain unclear.Apo-14 was amplified by a degenerate primer and then sequenced and analyzed using BLASTX,SignalP 3.0,NetNGlyc 1.0,YinOYang 1.2,NetPho 2.0,ClustalW,and MEGA 4.1 software.Sixty spec... |
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| Keywords: | marine biology Apo-14 clone sequence analysis salinity adaptation qRT-PCR |
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