Further Study on the Kinetics of Bioremediation of Soils Contaminated with the Herbicide 2,4‐Dichlorophenoxy Acetic Acid

The bioremediation of soil contaminated with the herbicide 2,4‐dichlorophenoxy acetic acid has been studied applying the bacterium Ralstonia eutropha. The effects of temperature, pH, and moisture content of soils on the rate of degradation of this herbicide have been investigated. The Taguchi experimental design method was applied to determine the relative impacts of the pertinent system variables. According to this approach, four series of experiments were performed under various operating conditions. In all four series the herbicide was degraded efficiently, however, the highest rate was observed at a temperature of 35°C, pH 6, and at 80% moisture content of the soil. A kinetic model for the biodegradation of the herbicide was developed for this particular conditions based on the Monod type growth equation and autocatalytic behavior of the decomposition reaction. A close correlation has been observed between the experimental data and those predicted from the model. The degree of agreement was between 85–95%.