Laser-Dye Imaging of the pH Field in a Laboratory Experiment |
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Authors: | Satoshi Someya Shigeru Bando Yongchen Song Baixin Chen Masahiro Nishio |
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Institution: | (1) National Institute of Advanced Industrial Science and Technology, Namiki, Tsukuba, Ibaraki 305-8564, Japan;(2) University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan |
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Abstract: | We established a precise DeLIF (Dual Emission Laser Light Fluorescence) system for the instant measurement of the pH field
in laboratory experiments. This method is very important in studies of the biological impacts of CO2 sequestration, i.e., effects of pH on organisms. The technique is demonstrated by applying it to the pH measurement around
a liquid CO2 droplet, which has a large gradient of CO2 concentration near its interface, the concentration value on the boundary being known as the solubility of CO2. In the Laser Induced Fluorescence (LIF) method using quinine and an ultraviolet laser light source, the relatively unstable
pulse of the laser source decreased the measurement reliability. We therefore investigated many kinds of fluorescent dye and
demonstrated the DeLIF measurement, identifying a process for cancelling fluctuations of laser light intensity. Quinine and
sulforhodamine were used as fluorescence dyes. The molar absorption coefficient and emission intensity of quinine depend on
the pH of aqueous solutions, while those of sulforhodamine are not as much affected by pH. The ratio of these fluorescence
intensities represents pH at each point of the image. As a result, the pH field was successfully measured with an uncertainty
of only approximately ±0.005, for 3.0 < pH.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Carbon dioxide laser induced fluorescence pH flow visualization dissolution |
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