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The complete DNA sequence of the mitochondrial genome of Sinonovacula constricta (Bivalvia: Solecurtidae)
Authors:ZHENG Runling  LI Jiale  NIU Donghong
Affiliation:1.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China2.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China;Aquaculture Division, E-Institute of Shanghai Universities, Shanghai 201306, China
Abstract:The nucleotide sequence of the mitochondrial genome of the solecurtidae Bivalvia mollusca Sinono-vacula constricta (GenBank accession number EU880278) has been determined and is reportedhere. We determined the complete mitochondrial genome sequence using long-PCR and Shot GunSequencing. Contained within the 17 225 base pairs (bp) are the two ribosomal RNA genes and12 protein coding genes typical of metazoan mitochondrial genomes. The S. constricta mitochon-drial DNA (mtDNA) did not contain a gene for atp8, similar to the mtDNA of Crassostrea virginica,Crassostrea giga and Mytilus edulis. The S. constricta mtDNA is 67.0% A+T (A 25.9%, C 10.5%, G22.5%, and T 41.1%). This value is higher than that for many invertebrate mitochondrial genomes.Only 19 putative tRNA genes are present in S. constricta and 27 noncoding regions, of which twoare large in size. The trnE and trnW genes as well as a second trnS were absent in S. constricta.The gene arrangement of S. constricta is different from the other Bivalvia genomes.
Keywords:Sinonovacula constricta   Mitochondrial DNA
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