入卵过程中太平洋牡蛎精子超微结构的变化

本文研究了精子入卵过程中太平洋牡蛎精子超微结构的变化规律。研究表明 ,精子入卵过程分为 4个时期 :顶体反应期、穿卵前期、穿卵期和融合期。顶体反应期 ,精子顶体物质释放 ,顶体致密部分向外延伸 ,形成 1层保护膜 ,亚顶体腔开始向顶端突起 ;穿卵前期 ,亚顶体腔前突形成顶体锥 ,并向卵膜释放亚顶体物质 ,精子外膜后移 ;穿卵期 ,精子核及线粒体进入卵膜形成的受精锥 ,线粒体数量减少 ,顶体突消失 ,精子外膜和尾部脱落 ;融合期 ,精子核进入卵内与质膜融合 ,核解体。

Ultrastructure Change of the Sperm of Crassostrea gigas During Egg Penetrating

The change of the sperm ultrastructure of Crassostrea gigas during egg penetrating was studied. The results show that this process can be divided into four periods: acrosome reaction, pre-penetrating, penetrating and fusion. During the period of acrosome reaction, the acrosome substances in the sperm are released, the dense part of acrosome elongates outward and forms a protective membrane. The space of sub-acrosome protrus toward the apex. During the period of pre-penetrating, the front part of protrusion of the sub-acrosome space forms the acrosome cone and the sub-acrosome substance is released to the egg membrane, and the plasma membrane of the sperm moves backward. During the period of penetration, the sperm nucleus and mitochondria enter the fertilization cone formed by the egg membrane, the amount of mitochondria reduces, the acrosomd cone disappears, and the outer membrane and tail part of the sperm slough. During the period of fusion, the sperm nucleus penetrates into the egg, fuses with the plasma, and disintegrates.