Methodological aspects of the determination of the Bacterioplankton number,biomass, and production |
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Authors: | N D Romanova A F Sazhin |
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Institution: | 1.Shirshov Institute of Oceanology,Russian Academy of Sciences,Moscow,Russia |
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Abstract: | The current methods used in world practice for the determination of the basic parameters of the vital functions of bacterioplankton
(the number, biomass, and production) are discussed. While comparing the different methods and their modifications, the peculiarities
of their application for particular natural or laboratory environments are emphasized. The specificity of applying different
microbiological methods for field research is examined. When choosing the methodological base for describing the characteristics
of a bacterial community, one should proceed from the specific problem facing the researcher. In the case of routine monitoring,
the following techniques are convenient and widely used: direct counts of DAPI fluorochrome-stained bacteria using a fluorescence
microscope; the calculation of the biomass of the microorganisms on the basis of the relationship between the cell’s mass
and its size; the estimation of the bacteria production via labeled leucine incorporation. |
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Keywords: | |
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