用RAPD方法对不同色三疣梭子蟹的分析

为寻找三疣梭子蟹体色相关的分子标记,本文用RAPD方法对紫色和茶绿色两种三疣梭子蟹基因组DNA进行体色相关遗传标记的筛选及其遗传多样性分析。从80个随机引物中共筛选了30个重复性好多态性高的引物,RAPD分析结果表明：紫色梭子蟹个体组成的群体多态性位点比例为61.29%,Nei氏基因多样性指数为0.3756,Shannon信息指数为0.5545;茶绿色梭子蟹个体组成的群体多态性位点比例为62.44%,Nei氏基因多样性指数为0.3451,Shannon信息指数为0.5099。紫色和茶绿色三疣梭子蟹之间Nei氏无偏差遗传相似性指数为0.9682,遗传距离为0.0323。这些结果表明,紫色和茶绿色三疣梭子蟹群体的遗传多样性程度接近,具有相似的遗传多样性基础。另外,有2个随机引物（SBSA09和SBSG16）扩增得到群体特异性片段各1条。

RAPD analysis between color-different crab individuals of Portunus trituberculatus

The blue swimming crabs Portunus trituberculatus with tea-green and/or purple carapace color have been discovered on the shore of eastern part of China these days. In this paper, 40 color-different crab individuals were evaluated using 80 RAPD primers. 30 primers were selected using bulk segregation analysis (BSA) and RAPD-PCR analysis. The results showed that the ratio of polymorphic loci was 61.29%, and Nei's gene diversity was 0.375 6, and Shannon's information index was 0.554 5 among purple crab individuals; and the ratio of polymorphic loci was 62.44%, and Nei's gene diversity was 0.3451, and Shannon's information index was 0.5099 among tea-green crab individuals. It was clear that the genetic diversity between purple and tea-green crabs was similar, which indicated close phylogenetic relationships between the purple and tea-green crabs. In addition, two primers (SBS A09 and SBS G16) got specific DNA bands by comparing forty individuals of color-different crab individuals through RAPD-PCR analysis and the following electrophoresis. They indicated close phylogenetic relationships between purple and tea-green crabs.