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北极海洋沉积物细菌和古菌群落结构分析
引用本文:林学政,张良,刘焱光,李阳.北极海洋沉积物细菌和古菌群落结构分析[J].海洋学报(英文版),2017,36(8):146-152.
作者姓名:林学政  张良  刘焱光  李阳
作者单位:国家海洋局第一海洋研究所, 青岛 266061,国家海洋局第一海洋研究所, 青岛 266061,国家海洋局第一海洋研究所, 青岛 266061,国家海洋局第一海洋研究所, 青岛 266061
基金项目:The Chinese Polar Environment Comprehensive Investigation and Assessment Program under contract No. CHINARE2014-03-05; the National Natural Science Foundation of China under contract No. 41176174; the Innovation and Development Regional Demonstration Program of Marine Economy under contract No. 12PYY001SF08-HYYS-1.
摘    要:This study was to investigate bacterial and archaeal community structure of pan-Arctic Ocean sediments by pyrosequencing.In total,investigation of three marine sediments revealed 15 002 bacterial and 4 362 archaeal operational taxonomic units(OTUs) at the 97% similarity level.Analysis of community structure indicated that these three samples had high bacterial and archaeal diversity.The most relatively abundant bacterial group in Samples CC1 and R05 was Proteobacteria,while Firmicutes was dominant in Sample BL03.Thaumarchaeota was the most relatively abundant archaeal phylum in Samples CC1 and R05,and the relative abundance of Thaumarchaeota was almost as high as that of Euryarchaeota in Sample BL03.These two phyla accounted for nearly 100% of the archaeal OTUs.δ-Proteobacteria and γ-Proteobacteria were the two most relatively abundant classes at Proteobacterial class level,and their relative abundance was more than 60% in Samples CC1 and R05.There were also differences in the top 10 relatively abundant bacterial and archaeal OTUs among the three samples at the 97% similarity,and only 12 core bacterial OTUs were detected.Overall,this study indicated that there were distinct microbial communities and many unique OTUs in these three samples.

关 键 词:北极沉积物  微生物群落  多样性  焦磷酸测序
收稿时间:2016/6/17 0:00:00

Bacterial and archaeal community structure of pan-Arctic Ocean sediments revealed by pyrosequencing
LIN Xuezheng,ZHANG Liang,LIU Yanguang and LI Yang.Bacterial and archaeal community structure of pan-Arctic Ocean sediments revealed by pyrosequencing[J].Acta Oceanologica Sinica,2017,36(8):146-152.
Authors:LIN Xuezheng  ZHANG Liang  LIU Yanguang and LI Yang
Institution:The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
Abstract:This study was to investigate bacterial and archaeal community structure of pan-Arctic Ocean sediments by pyrosequencing. In total, investigation of three marine sediments revealed 15 002 bacterial and 4 362 archaeal operational taxonomic units (OTUs) at the 97% similarity level. Analysis of community structure indicated that these three samples had high bacterial and archaeal diversity. The most relatively abundant bacterial group in Samples CC1 and R05 was Proteobacteria, while Firmicutes was dominant in Sample BL03. Thaumarchaeota was the most relatively abundant archaeal phylum in Samples CC1 and R05, and the relative abundance of Thaumarchaeota was almost as high as that of Euryarchaeota in Sample BL03. These two phyla accounted for nearly 100% of the archaeal OTUs. δ-Proteobacteria and γ-Proteobacteria were the two most relatively abundant classes at Proteobacterial class level, and their relative abundance was more than 60% in Samples CC1 and R05. There were also differences in the top 10 relatively abundant bacterial and archaeal OTUs among the three samples at the 97% similarity, and only 12 core bacterial OTUs were detected. Overall, this study indicated that there were distinct microbial communities and many unique OTUs in these three samples.
Keywords:Arctic sediment  microbial community  diversity  pyrosequencing
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