| THE INSEMINATION OF GOLDFISH(CARASSIUM AURATUS) OOCYTE MATURED IN VITRO |
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| 作者姓名： | 王壬学 吴贤汉 周晶 张士璀 马英杰 吴尚懃 史瀛仙 |
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| 作者单位： | Institute of Oceanology，Academia Sinica，Qingdao，266071，Institute of Oceanology，Academia Sinica，Qingdao，266071，Institute of Oceanology，Academia Sinica，Qingdao，266071，Institute of Oceanology，Academia Sinica，Qingdao，266071，Institute of Oceanology，Academia Sinica，Qingdao，266071，Institute of Oceanology，Academia Sinica，Qingdao，266071，Institute of Developmental Biology，Academia Sinica，Beijing，100080 |
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| 摘 要： | Full maturation of goldfish oocyte was induced in vitro by 17 a-hydroxy-20B-dihydroprogesterone. The oocyte maturation involves GV migration to the periphery of the oocyte and germinal vesicle breakdown (GVBD). In the experiment, incubation duration for GVBD varied in different broods of oocytes. Generally, if the duration for GVBD was shorter than 6 h, oocytes would have a better chance to survive after maturation and insemination. The maturation of nucleus (GV) and cytoplasm are not synchronous. Cytoplasm maturation occurs several hs after GVBD. Oocytes inseminated 8-9 h after GVBD have the highest fertilizing and hatching rate. Fertilized ova matured in vitro can develop to sexually mature adults capable of reproduction.
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