| Molecular genetic diversity of bacteria in the bottom section of arctic sea ice from the Canada Basin |
| |
| 作者姓名： | LI Huirong YU Yong CHEN Bo ZENG Yinxin REN Daming |
| |
| 作者单位： | LI Huirong1，YU Yong1，CHEN Bo1，ZENG Yinxin1，REN Daming2 1. State Oceanic Administration Key Laboratory for Polar Science，Polar Research Institute of China，Shanghai 200136，China 2. State Key Laboratory of Genetic Engineering，Institute of Genetics，Fudan University，Shanghai 200433，China |
| |
| 基金项目： | the National Basic Research Program of China under coutract，the Science and Technology Basic Work Program of China under coutract，中国科学院资助项目，中国北极科学考察基金，the Ministry of Finance of China and organized by the Chinese Arctic and Antarctic Administration (CAA)，The participants in this joint work are from the institutions (e.g. |
| |
| 摘 要： | PCR-DGGE approach was used to analyze bacterial diversity in the bottom section of seven arctic sea ice samples colleted from the Canada Basin. Thirty-two 16S rDNA sequences were obtained from prominent DGGE bands. The closest relatives of these sequences are found to be those of cultivated or uncultured bacteria from antarctic or arctic sea ice. Phylogenetic analysis clustered these sequences or phylotypes within α- proteobacteria, γ-proteobacteria and CFB (cytophaga-flexibacter-bacteroides) group. Sequences belonging to γ-proteobacteria were dominant and members of the CFB group were highly abundant. It was suggested that the CFB group was the representative of the bottom section of sea ice samples.
|
| 关 键 词： | sea ice Arctic Ocean bacteria 16S rDNA phylogenetic analysis |
| 收稿时间： | 2005/7/26 0:00:00 |
| 修稿时间： | 2005/10/23 0:00:00 |
Molecular genetic diversity of bacteria in the bottom section of arctic sea ice from the Canada Basin |
| |
| LI Huirong,YU Yong,CHEN Bo,ZENG Yinxin,REN Daming.Molecular genetic diversity of bacteria in the bottom section of arctic sea ice from the Canada Basin[J].Acta Oceanologica Sinica,2005,24(6):153-161. |
| |
| Authors: | LI Huirong YU Yong CHEN Bo ZENG Yinxin and REN Daming |
| |
| Institution: | 1.State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China2.State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China |
| |
| Abstract: | PCR-DGGE approach was used to analyze bacterial diversity in the bottom section of seven arctic sea ice samples colleted from the Canada Basin. Thirty-two 16S rDNA sequences were obtained from prominent DGGE bands. The closest relatives of these sequences are found to be those of cultivated or uncultured bacteria from antarctic or arctic sea ice. Phylogenetic analysis clustered these sequences or phylotypes within α- proteobacteria, γ-proteobacteria and CFB (cytophaga-flexibacter-bacteroides) group. Sequences belonging to γ-proteobacteria were dominant and members of the CFB group were highly abundant. It was suggested that the CFB group was the representative of the bottom section of sea ice samples. |
| |
| Keywords: | sea ice Arctic Ocean bacteria 16S rDNA phylogenetic analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《海洋学报(英文版)》浏览原始摘要信息 |
| 点击此处可从《海洋学报(英文版)》下载免费的PDF全文 |
