| 海带雌性原生质体的分离与培养 |
| |
| 引用本文： | 秦松,王希华,童顺,曾呈奎.海带雌性原生质体的分离与培养[J].海洋与湖沼,1995,26(S1):126-129. |
| |
| 作者姓名： | 秦松 王希华 童顺 曾呈奎 |
| |
| 作者单位： | 中国科学院海洋研究所 青岛 266071;中国科学院海洋研究所 青岛 266071;中国科学院海洋研究所 青岛 266071;中国科学院海洋研究所 青岛 266071 |
| |
| 基金项目： | 国家科委资助;山东省科委合同攻关项目 |
| |
| 摘 要： | 于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。
|
| 关 键 词： | 海带 雌配子体 原生质体 鲍酶 纤维素酶 |
| 收稿时间： | 1994/4/11 0:00:00 |
| 修稿时间： | 7/8/1994 12:00:00 AM |
ISOLATION AND CULTURE OF FEMALE HAPLOID PROTOPLASTS FROM LAMINARIA JAPONICA |
| |
| Qin Song,Wang Xihu,Tong Shun and Zeng Chengkui.ISOLATION AND CULTURE OF FEMALE HAPLOID PROTOPLASTS FROM LAMINARIA JAPONICA[J].Oceanologia Et Limnologia Sinica,1995,26(S1):126-129. |
| |
| Authors: | Qin Song Wang Xihu Tong Shun and Zeng Chengkui |
| |
| Institution: | Institute of Oceanology, Academia Sinica, Qingdao 266071;Institute of Oceanology, Academia Sinica, Qingdao 266071;Institute of Oceanology, Academia Sinica, Qingdao 266071;Institute of Oceanology, Academia Sinica, Qingdao 266071 |
| |
| Abstract: | Female haploid protoplasts were isolated in January to April, 1993 from female gametophytes of Laminaria japonica using abalone enzymes extracted from the digestive organs of the abalone Haliotis discus hannai. Single cells and protoplasts could be isolated effectively from filamentous gametophytes after incubation with 1% (W / V) abalone enzymes and 2%(W / V) cellulase (Onozyka R-10) at 25°C. Microscopy and fluorescent microscopy revealed that viable female haploid protoplasts were isolated, and that cell walls were regenerated after 2 days. |
| |
| Keywords: | Laaninaria japonica Female gametophyte Protoplast Abalone enzymes Cellulase |
|
| 点击此处可从《海洋与湖沼》浏览原始摘要信息 |
| 点击此处可从《海洋与湖沼》下载免费的PDF全文 |
