真鲷(Pagrosomus major)肠上皮组织中类立克 次体的超微形态与细胞病理学的初步研究

运用电镜技术对青岛地区患病真鲷进行了病原体检测，在肠上 皮组织细胞中发现一种类立克次体病原体。研究表明，该病原体具有完整的细胞包膜, 内部 具有低电子密度拟核，在胞浆中以两种类型存在-繁殖型和静止型；繁殖型类立克次体呈圆 形、椭圆、哑铃、长棒状，其长轴为0.2～0.8μm，形成包涵体，以分裂方式进行增殖；静止 型类立克次体为圆形，中等电子密度，直径为40～70nm，由繁殖型类立克次体出芽增殖形成 ；线粒体参与了包涵体的形成和类立克次体的增殖过程。细胞病理变化为上皮细胞肿胀并部 分解体，微绒毛破坏，线粒体变形，部分内嵴溶解；内质网膨胀，核糖体脱落。类立克次体 是导致鱼类病害的重要病原体。

Preliminary Study on the Ultrastructure and Cell Pathology of Rickettsia-like Organism in Intestine Epithelial Tissue of Pagrosomus Major

The Rickettsia-like organism (RLO) in the enteric epi thelium of the diseased Pagrosomus major ［Temmincket Schlegel］ were observ ed with electron microscopy. The results showed that the RLO with an integrated cell membrane and a nucleoid of low electronic density appeared in the cytoplasm . It had two forms, the Vegetative and the Vesting forms. The Vegetative form ha d the shapes in rotundity, ellipse dumbbell and long stick. The length of the Ve getative form was 0.2～0.8μm. It formed inclosion body in cytoplasm and was repr oduced by cell division. The Vesting form with shape of rotundity had medium ele ctronic density and was 40～70nm in diameter. It was derived from the Vegetative form by budding. The Mitochondria was involved in the formation of inclusion bod y and the proliferations. The cell pathologic changes were epithelial cell swell ing, microvilli destruction, mitochondria deformation, endocrista dissolvation, endoplasmic reticulum expansion and ribosome disattachment. Rickettsia-like org anism was an important pathogen which caused the fish disease.