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| 应用蛋白质组学技术对寄生压力下缢蛏血淋巴的抗性研究 | |
| 引用本文： | 张鹭,彭宣宪,陈寅山.应用蛋白质组学技术对寄生压力下缢蛏血淋巴的抗性研究[J].海洋湖沼通报,2004(2):80-86. |
| 作者姓名： | 张鹭 彭宣宪 陈寅山 |
| 作者单位： | 1. 齐齐哈尔大学生命科学院,黑龙江,齐齐哈尔,161005 2. 厦门大学生命科学院,厦门,361005 3. 福建师范大学生物工程学院,福建,福州,350007 |
| 摘 要： | 以闽、浙一带重要的海产养殖贝类缢蛏(Sinonovacula constricta Lamarck)为研究对象，应用蛋白质组学方法探求其在受寄生压力下血淋巴内40KDa以下小分子蛋白质组的变化，寻找缢蛏血淋巴的抗性机制，明确血淋巴在机体防御体系中的作用。在寄生压力下，缢蛏血淋巴内有多种蛋白发生变化，包括生长调节蛋白，金属硫蛋白-B．唾液酸结合凝集素，凝血因子IXA轻链等，涉及多种与炎症反应，神经营养，基因表达调控．蛋白质合成等有关的功能。说明血淋巴在缢蛏的防御体系中具有关键性作用。 |
| 关 键 词： | 海产养殖 贝类 缢蛏 蛋白质组学 血淋巴 寄生 小分子蛋白质 |
| 文章编号： | 1003-6482(2004)02-0080-07 |
| 修稿时间： | 2003年10月21 |
STUDY OF RESISTANCE IN HEMOLYMPH OF Sinonovacula constricta UNDER PARASITIZING WITH PROTEOMIC APPROACHES |
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| ZHANG Lu ,PENG Xuanxian ,CHEN Yinshan.STUDY OF RESISTANCE IN HEMOLYMPH OF Sinonovacula constricta UNDER PARASITIZING WITH PROTEOMIC APPROACHES[J].Transaction of Oceanology and Limnology,2004(2):80-86. | |
| Authors: | ZHANG Lu PENG Xuanxian CHEN Yinshan |
| Institution: | ZHANG Lu 1,PENG Xuanxian 2,CHEN Yinshan 3 |
| Abstract: | Sinonovacula constricta (Lamarck), a kind of mollusks, is important in marine aquaculture due to its high market demand. This study used proteomic techniques to detect the parasitism-specific proteins in hemolymph under long-term parasitizing and then identified seven proteins, including Growth regulating protein,Metallothionein-B,Sialic acid-binding lectin and Factor IXA light chain etc, which have multiple functions such as anti-inflammation, nutrition, gene expression and regulation, and protein synthesis. It is obvious that hemolymph is critical in S. constricta defense mechanism. |
| Keywords: | Proteomic approach Sinonovacula constricta (Lamarck) parasitism hemolymph small protein |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |