利用两种活体荧光技术分析北极微藻对光照变化的响应

采用调制叶绿素荧光技术（脉冲-振幅-调制：Pulse-Amplitude-Modulation，简称为PAM技术）和荧光光谱两种技术研究不同光照下的两种北极微藻（北极异帽藻（Heterocapsa arctica）和红胞藻（Rhodomonas sp.）的荧光特性，发现（1）两种藻在三个不同光照水平下（5 000 lux、15 000 lux、25 000 lux）光合生理变化特征相似：随着光照强度增加，光量子产量越低，光合效率越低；在15 000 lux和25 000 lux两个较强光照条件下，最大光合电子传递速率（rETRmax）没有提高，且捕光能力下降；25 000 lux光照强度下，均处于胁迫状态，但对强光的耐受能力增强；（2）两种藻在15 000 lux条件下Chl a荧光强度最高，25 000 lux条件下的Chl a荧光强度都显著降低，受到不同程度的光损伤；类胡萝卜素和多甲藻素是维持北极异帽藻15 000 lux下种群生存的有效色素；藻胆素和Chl c则分别为维持红胞藻5 000 lux和15 000 lux条件下的种群的生存起了积极作用。

STUDIES ON RESPONSE OF ARCTIC MICROALGAE TO CHANGE OF IRRADIANCE,APPLYING TWO KINDS OF FLUORESCENT TECHNIQUES

This work investigatedfluorescence characteristic of two kinds of arctic algae (Heterocapsa arctica and Rhodomonas sp.) by two kinds of fluorescence techniques in vivo including Pulse-Amplitude-Modulation (PAM) and fluorescence spectrum. The main results are as follows: (1) the photosynthetic physiology of two kinds of algae were similar under the three kinds of irradiance (5000 lux 15000 lux and 25000 lux); the irradiance is stronger, the value of the Fv/Fm and photosynthetic efficiency are higher; under 25000 lux, they were in stress state, but their endurance capacity was enhanced. (2) the fluorescence intensity of Chl a of the two kinds of algae is highest under 15000lux; the fluorescence intensity of Chl a reduced on 15000lux; Carotenoids and Peridinins were the effective pigments for the survival of Heterocapsa arctica under 15000lux; phycobilin and Chl c had a positive effect on maintain the existence of Rhodomonas sp. under 5000 lux and 15000 lux.