| Preliminary study on mitochondrial 16S rRNA gene sequences and phylogeny of flatfishes （Pleuronectiformes） |
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| 引用本文： | 尤锋,刘静,张培军,相建海.Preliminary study on mitochondrial 16S rRNA gene sequences and phylogeny of flatfishes （Pleuronectiformes）[J].中国海洋与湖沼学报,2005,23(3):335-339. |
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| 作者姓名： | 尤锋 刘静 张培军 相建海 |
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| 作者单位： | InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China |
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| 基金项目： | Supported by Natural Science Fund of China (No. 30271036) and Natural Science Fund of Shandong Province of China |
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| 摘 要： | A 605 bp section of mitochondrial 16S rRNA gene from Paralichthys olivaceus, Pseudorhombus cinnamomeus, Psetta maxima and Kareius bicoloratus, which represent 3 families of Order Pleuronectiformes was amplified by PCR and sequenced to show the molecular systematics of Pleuronectiformes for comparison with related gene sequences of other 6 flatfish downloaded from GenBank. Phylogenetic analysis based on genetic distance from related gene sequences of 10 flatfish showed that this method was ideal to explore the relationship between species, genera and families. Phylogenetic trees set-up is based on neighbor-joining, maximum parsimony and maximum likelihood methods that accords to the general rule of Pleuronectiformes evolution. But they also resulted in some confusion. Unlike data from morphological characters, P olivaceus clustered with K.bicoloratus, but P cinnamomeus did not cluster with t3. olivaceus, which is worth further studying.
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| 关 键 词： | 比目鱼 线粒体 RNA 基因 |
| 收稿时间： | 12 October 2003 |
| 修稿时间： | 10 April 2005 |
Preliminary study on mitochondrial 16S rRNA gene sequences and phylogeny of flatfishes (Pleuronectiformes) |
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| You Feng,Liu Jing,Zhang Peijun,Xiang Jianhai.Preliminary study on mitochondrial 16S rRNA gene sequences and phylogeny of flatfishes (Pleuronectiformes)[J].Chinese Journal of Oceanology and Limnology,2005,23(3):335-339. |
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| Authors: | You Feng Liu Jing Zhang Peijun Xiang Jianhai |
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| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China |
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| Abstract: | A 605 bp section of mitochondrial 16S rRNA gene from Paralichthys olivaceus, Pseudorhombus cinnamomeus, Psetta maxima and Kareius bicoloratus, which represent 3 families of Order Pleuronectiformes was amplified by PCR and sequenced to show the molecular systematics of Pleuronectiformes for comparison with related gene sequences of other 6 flatfish downloaded from GenBank. Phylogenetic analysis based on ge- netic distance from related gene sequences of 10 flatfish showed that this method was ideal to explore the rela- tionship between species, genera and families. Phylogenetic trees set-up is based on neighbor-joining, maximum parsimony and maximum likelihood methods that accords to the general rule of Pleuronectiformes evolution. But they also resulted in some confusion. Unlike data from morphological characters, P. olivaceus clustered with K. bicoloratus, but P. cinnamomeus did not cluster with P. olivaceus, which is worth further studying. |
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| Keywords: | mtDNA 16s rRNA gene sequences phylogeny Pleuronectiformes |
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