Effect of Nitrogen Sources on the Growth and Docosahexaenoic Acid Accumulation in Crypthecodinium cohnii

The effect of various nitrogen sources on the growth and docosahexaenoic acid (DHA) yield is determined in Cryphecodinium cohnii ATCC30556,Single nitrogen tryptone and peptone are suitable to growth,the dry weight biomass is up to 2.78g/L and 2.70g/L respectively on medium containing 0.34g/L nitrogen.Peptone is a favorable nitrogen source for DHA accumulation,DNA yield increases up to 338.56mg/L.Using peptone and KNO3 as a multiple nitrogen source,the highest biomass and DHA yield are obtained from medai containing 0.34g/L nitrogen in which the ratio of peptone-N:NO3^--N is 1:2 ,and the bionmass and DHA yield are 2.98g/L(DW) and 527.97mg/L respectively.

Effect of Nitrogen Sources on the Growth and Docosahexaenoic Acid Accumulation in Crypthecodinium cohnii

The effect of various nitrogen sources on the growth and docosahexaenoic acid (DHA) yield is determined in Crypthecodinium cohnii ATCC30556. Single nitrogen tryptone and peptone are suitable to growth, the dry weight biomass is up to 2.78 g/L and 2.70g/L respectively on medium containing 0.34 g/L nitrogen. Peptone is a favorable nitrogen source for DHA accumulation, DHA yield increases up to 338.56 mg/L. Using peptone and KNO3 as a multiple nitrogen source, the highest biomass and DHA yield are obtained from media containing 0.34 g/L nitrogen in which the ratio of peptone-N:NO3--N is 1:2,and the biomass and DHA yield are 2.98 g/L(DW) and 527.97mg/L respectively.