Phylogenetic diversity of Type I polyketide synthase genes from sediments of Ardley Island in Antarctica |
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Authors: | ZHAO Jing YANG Ning CHEN Xinglin JIANG Qingru and ZENG Runying |
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Institution: | 1.Department of Marine Technology and Ocean Engineering, College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China2.Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China |
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Abstract: | The diversity of modular polyketide synthase (PKS) genes in sediments of Ardley Island in Antarctica, was studied by restriction
fragment length polymorphism (RFLP) analysis. Phylogenetic analysis of 14 amino acid (AA) sequences indicates that the identified
ketosynthase (KS) domains were clustered with those from diverse bacterial groups, including Cyanobacteria, γ-Proteobacteria, Actinobacteria, Firmicutes, and some unidentified microorganisms from marine sponge, bryozoan and other environmental samples. The obtained KS domains
showed 43%-81% similarity at the AA level to reference sequences in GenBank. Six identified KS domains showed diverse sequences
of the motif (VQTACSTS) that was used to identify the hybrid PKS/nonribosomal peptide synthetase (NRPS) enzyme complex, and
formed a new branch. These results reveal a high diversity and novelty of PKS genes in antarctic sediments. |
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Keywords: | polyketide synthase diversity Antarctica |
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