海带渣资源化利用及在小球藻培养中的应用研究

海带渣是海带加工中的固体废弃物,将其资源化利用生产高附加值产品具有重要意义.文章以海带渣为原料,糖化液的总还原糖质量浓度、单糖组成和小球藻培养效果为主要评估指标,探究不同预处理方法对海带渣酶解的影响,以及海带渣资源化利用培养微藻工艺的可行性.结果 显示:(1)粗纤维和灰分是海带渣主要组成成分;(2)常温常压稀硫酸预处理...

RESOURCE UTILIZATION OF KELP RESIDUE AND APPLICATION IN CHLORELLA CULTURE

Kelp residue is a type of solid waste from kelp processing. It is of great significance to utilize it to produce high value-added products. Therefore, kelp residue and was used as raw material and saccharified. The total reducing sugar concentration, monosaccharide composition, and Chlorella cultivation effect of the saccharification liquid were used as the main indexes to evaluate the effects of different pretreatment methods on enzymatic hydrolysis of kelp residue, and the feasibility of kelp residue resource utilization and cultivation of microalgae. The results show that:(1) crude fiber and ash are the main components of kelp residue.(2) After pretreatment with dilute sulfuric acid at room temperature and atmospheric pressure, the total reducing sugar concentration of saccharification solution was increased from 2.7 to 26.3 g/L.Under the optimal saccharification conditions(cellulase dosage 50 IU/g, enzymolysis time 36 h, solid-liquid ratio 1:5), the total reducing sugar concentration of saccharification solution reached(53.4±0.3) g/L, the yield of total reducing sugar was 98.9%.(3) The monosaccharide of saccharification solution included mainly glucose, and its mass concentration was 48.0 g/L.(4) The biomass(0.93 g/L) of Chlorella sp. scsio-559 cultured in 2 g/L saccharification solution was significantly higher than that of autotrophic group and 2 g/L glucose group, which is conducive to the accumulation of polyunsaturated fatty acids. Therefore, the saccharified liquid of kelp residue has obvious advantages for Chlorella cultivation, and is potential to be used as an organic carbon source for microalgae cultivation. This study provided a data support for better resource utilization of kelp residue, and a theoretical basis for improving high value application of kelp residue while reducing production cost and environmental pollution in the microalgae industry.