基于低压变频电场技术对带鱼(Trichiurus lepturus)保鲜过程中微生物群落影响分析

带鱼(Trichiurus lepturus)在低温条件运输和贮藏过程中,易受微生物和内源性蛋白酶的影响而产生胺类和醛类物质导致腐败。为了研究低压变频电场对微冻带鱼在贮藏过程中的微生物群落特征,采用Miseq基因组测序技术,对贮藏至40d的舟山带鱼的微生物群落进行碱基信息测序。将产生的碱基序列通过OTU聚类、多样性分析、相关性分析等手段分析了影响带鱼保鲜效果的微生物群落组成和发育信息。在此基础上,研究了不同强度电场(0,2,2.5,3 kV/m)处理对带鱼微生物群落组成的影响。根据菌属相对丰度结果,推测希瓦氏菌属(Shewanella)、假单胞菌属(Pseudomonas)、紫色杆菌属(Janthinobacterium)是带鱼微生物腐败主要致腐菌。结果表明,40 d后,经3 kV/m处理的样品其OTU6嗜冷菌属和OTU13假单胞菌属c的相对丰度均小于1%,而且OTU7、OTU14、OTU8、OTU9等菌属未被OTU聚类。结果表明,经3kV/m电场强度的低压静电场处理的抑菌保鲜效果最好,表现为在贮藏过程中杂菌种类最少,样本微生物群落物种丰度和多样性均最低,腐败程度最低。

EFFECT OF LOW-VOLTAGE ALTERNATING FREQUENCY ELECTRIC FIELD ON MICROBIAL COMMUNITY OF HAIRTAIL TRICHIURUS LEPTURUS DURING PRESERVATION

To study the effect of low-voltage variable-frequency electric field on the microbial community characteristics of microfrozen hairtail(Trichiurus lepturus)during storage,Miseq genome sequencing technology was used to sequence the base information of the microbial community of Zhoushan hairtail stored for 40 days.The microbial community composition and development information affecting the preservation effect of hairtail were analyzed by OTU clustering,diversity analysis,and correlation analysis.On this basis,the effects of electric field in different intensities(0,2,2.5,3 kV/m)on the microbial community composition of hairtail were studied.According to the results of relative abundance of bacteria,we speculated that Shewanella,Pseudomonas,and Janthinobacterium were the main spoilage bacteria of hairtail microbial spoilage.The results show that after 40-day storage,the relative abundances of OTU6 psychrophilic bacteria and OTU13 Pseudomonas C were less than 1%,and OTU7,OTU14,OTU8,OTU9 and other bacterial genera were not clustered by OTU.Specifically,the antibacterial and fresh-keeping effect of low-voltage electrostatic field in 3 kV/m intensity was the best,under which the species of miscellaneous bacteria were the least,the species abundance and diversity of microbial community were the lowest,and the degree of corruption was the lowest.