钝顶螺旋藻藻胆体的简易制备及褐藻酸钠对其稳定性的保护作用

以钝顶螺旋藻为材料，用改进的简易方法分离得到完整藻胆体，室温最大荧光发射峰位于６８１ｎｍ，加水解离后最大荧光发射蓝移到６５５ｎｍ，Ｆ６８０有两个峰，分别位于６３８ｎｍ和６５０ｎｍ，从而表明藻胆是完整的。用４３６ｎｍ波长激发，没有出现Ｃｈｌａ的特征荧光峰，Ｆ７２０的荧光激发光谱，也没有出现Ｃｈｌａ的峰，表明藻胆体溶液中不含Ｃｈｌａ。在藻胆体溶液中加入１％的褐藻酸钠，室温下保存１５ｄ后，光谱特性没有变

ISOLATION OF PHYCOBILLSMES IN Spirulina platensis WITH A SIMPLE METHOD AND ROLE OF SODIUM ALGINATE IN PROTECTION OF PHYCOBILISOME STABILITY

The phycobilisomes in Spirulina platensis were isolated with a simple method, the maximum fluorescence emission of the phycobilisomes at room temperature was at 681 nm, 655 nm after dissociation in water, which indicated that the isolated phycobilisomes were intact. Moreover, there was no Chla in the phycobilisome solution. When 1 % sodium alginate was added into the phycobilisome solution and was stored for 15 days at room temperature, there were no changes in the fluorescence spectra, which suggested that the phycobilisomes still kept intact. The results showed that 1 % sodium alginate in the phycobilisome solution was helpful to prevent phycobilisome dissociation, and the mechanism was also discussed.