Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography |
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| Authors: | Guoyan Ren Bafang Li Xue Zhao Yongliang Zhuang Mingyan Yan Hu Hou Xiukun Zhang Li Chen |
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| Institution: | (1) Food and Bioengineering Department, Henan University of Science and Technology, Luoyang, 471003, P. R. China;(2) College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, P. R. China |
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| Abstract: | In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified
target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted
against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions
containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted
water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q
water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method
was the optimum extraction method selected by HPLC. |
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| Keywords: | HPLC glycoprotein jellyfish extraction method |
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