Quantifying hydrogen peroxide in iron-containing solutions using leuco crystal violet |
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Authors: | Email author" target="_blank">Corey?A?CohnEmail author Aimee?Pak Daniel?Strongin Martin?A?Schoonen |
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Institution: | (1) Department of Geosciences and Center for Environmental Molecular Science, Stony Brook University, Stony Brook, New York, 11794-2100;(2) Department of Chemistry and Center for Environmental Molecular Science, Stony Brook University, Stony Brook, New York, 11794-2100;(3) Department of Chemistry, Temple University, Philadelphia, Pennsylvania 19122 and Center for Environmental Molecular Science, Stony Brook University, Beury Hall 201, 1901 North 13th Street, Stony Brook, New York, 11794-2100;(4) Department of Geosciences and Center for Environmental Molecular Science, Stony Brook University, Stony Brook, New York, 11794-2100 |
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Abstract: | Hydrogen peroxide is present in many natural waters and wastewaters. In the presence of Fe(II), this species decomposes to
form hydroxyl radicals, that are extremely reactive. Hence, in the presence of Fe(II), hydrogen peroxide is difficult to detect
because of its short lifetime. Here, we show an expanded use of a hydrogen peroxide quantification technique using leuco crystal
violet (LCV) for solutions of varying pH and iron concentration. In the presence of the biocatalyst peroxidase, LCV is oxidized by hydrogen peroxide, forming a colored
crystal violet ion (CV+), which is stable for days. The LCV method uses standard equipment and allows for detection at the low microM concentration
level. Results show strong pH dependence with maximum LCV oxidation at pH 4.23. By chelating dissolved Fe(II) with EDTA, hydrogen peroxide can be stabilized for analysis. Results are presented for
hydrogen peroxide quantification in pyrite–water slurries. Pyrite–water slurries show surface area dependent generation of
hydrogen peroxide only in the presence of EDTA, which chelates dissolved Fe(II). Given the stability of CV+, this method is particularly useful for field work that involves the detection of hydrogen peroxide. |
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