Molecular diversity of Thermococcales isolated from Guaymas Basin hydrothermal vents |
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| Authors: | LIU Lupeng WANG Fengping XU Jun and XIAO Xiang |
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| Institution: | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology;State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology;State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;School of SystemBiology, Shanghai Jiao Tong University, Shanghai 200240, China;State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology;State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology |
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| Abstract: | Members of Thermococcales are organoheterotrophic hyperthermophilic anaerobes consisting of three genera, Thermococcus, Pyrococcus, and Palaeococcus, among which Thermococcus are the most frequently isolated. In this study, a variety of Thermococcales strainswere isolated fromthe Guaymas Basin hydrothermal vents under different temperatures and pressures. Based on their 16S rRNA gene sequences, all the strains isolated from85°C or 95°C enrichment cultures at ambient pressure were classified as Thermococcus, while strains isolated from108°C-30MPa enrichment cultures belonged to Pyrococcus. The combination of high pressure and high temperature for enrichment was shown to be an efficientmethod for isolating Pyrococcus strains. Through selecting and analyzing withmultiplemolecular phylogeneticmarkers, the isolated Thermococcales strains were found quite diversified including several putative novel species. This demonstrates a vast genetic reservoir of Thermoccoccales in the deep-sea hydrothermal vents, and a fast evolving of the Thermococcales species adapting to the fast changing environment. |
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| Keywords: | hydrothermal vents Thermococcales Guaymas Basin |
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