| Variation in the immunoglobulin levels in turbot （Scophthalmus maximus） after vaccination with Streptococcus iniae |
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| 引用本文： | 战文斌,刘洪明,邢婧,绳秀珍,唐小千.Variation in the immunoglobulin levels in turbot （Scophthalmus maximus） after vaccination with Streptococcus iniae[J].中国海洋与湖沼学报,2009,27(3):536-542. |
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| 作者姓名： | 战文斌 刘洪明 邢婧 绳秀珍 唐小千 |
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| 作者单位： | Laboratory of Pathology and Immunology of Aquatic Animals, LMMEC, Ocean University of China, Qingdao 266003, China |
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| 基金项目： | the National Basic Research Program of China，the High Technology Research and Development Program of China，the National Natural Science Fundation of China |
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| 摘 要： |
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| 关 键 词： | 酶联免疫吸附测定 大菱鲆 链球菌 接种 蛋白水平 表面等离子体 ELISA 免疫球蛋白 |
Variation in the immunoglobulin levels in turbot (<Emphasis Type="Italic">Scophthalmus maximus</Emphasis>) after vaccination with <Emphasis Type="Italic">Streptococcus iniae</Emphasis> |
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| Wenbin?Zhan,Hongming?Liu,Jing?Xing,Xiuzhen?Sheng,Xiaoqian?Tang.Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae[J].Chinese Journal of Oceanology and Limnology,2009,27(3):536-542. |
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| Authors: | Wenbin Zhan Hongming Liu Jing Xing Xiuzhen Sheng Xiaoqian Tang |
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| Institution: | Laboratory of Pathology and Immunology of Aquatic Animals, LMMEC, Ocean University of China, Qingdao 266003, China |
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| Abstract: | A pathogenic bacterium(S636),identified as Streptococcus iniae,was isolated from turbot(Scophthalmus maximus) in 2005.We immunized turbot with formalin-killed S.iniae four times(on days 1,14,21,and 28) by intraperitoneal inoculation.After each vaccination,we obtained serum samples and isolated the lymphocytes from the peripheral blood,spleen,pronephros,and mesonephros.We measured surface Ig-positive(sIg+) lymphocytes and serum antibody levels from these organs using flow cytometry and enzyme-linked immunoso... |
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| Keywords: | turbot Streptococcus iniae flow cytometry immunoglobulin |
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