| 金龟子绿僵菌甘露糖6-磷酸异构酶基因cDNA序列的克隆及分析 |
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| 引用本文： | 李亚.金龟子绿僵菌甘露糖6-磷酸异构酶基因cDNA序列的克隆及分析[J].广东海洋大学学报,2011(4). |
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| 作者姓名： | 李亚 |
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| 作者单位： | 广东海洋大学农学院; |
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| 基金项目： | 广东省自然科学基金(9452408801002439); 湛江市科技计划项目 |
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| 摘 要： | 通过绿僵菌属甘露糖6-磷酸异构酶基因保守核苷酸区域设计简并性引物,采用RT-PCR及RACE-PCR技术成功克隆了金龟子绿僵菌mpi基因cDNA序列。该基因cDNA序列全长为1 513 bp,开放阅读框长度为1 328 bp,共编码441氨基酸。BLAST分析发现该基因演绎的氨基酸序列与其它真菌同源性较高,蛋白结构分析表明MPI蛋白是较保守的蛋白磷酸酶结构特征,主要由α螺旋和不规则卷曲构成。
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| 关 键 词： | 金龟子绿僵菌 RACE 甘露糖6-磷酸异构酶基因 克隆 |
Cloning and Sequence Analysis of Mannose-6-phosphate Isomerase cDNA from Metarhizium anisopliae ZJ1109 |
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| LI Ya.Cloning and Sequence Analysis of Mannose-6-phosphate Isomerase cDNA from Metarhizium anisopliae ZJ1109[J].Journal of Zhanjiang Ocean University,2011(4). |
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| Authors: | LI Ya |
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| Institution: | LI Ya(Agricultural College,Guangdong Ocean University,Zhanjiang 524088,China) |
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| Abstract: | The primers were designed according to the conservative sequence of mannose-6-phosphate isomerase gene,and the complete cDNA sequence of Metarhizium anisopliae was amplified by RT-PCR and RACE PCR.The complete cDNA sequence of mpi gene was 1513 bp and the complete ORF length was 1328 bp which encoded a protein with 441 amino acid residues.Blast analysis indicated the deduced amino acid sequence of the mpi gene from M.anisopliae showed high homology with other fungi respectively.The analysis of protein struc... |
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| Keywords: | Metarhizium anisopliae RACE mannose-6-phosphate isomerase gene cloning |
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