Seasonal variation of hepatic mixed function oxidases in winter flounder (Pseudopleuronectes americanus)

Indices of hepatic mixed function oxidase (MFO) activity in winter flounder (Pseudopleuronectes americanus) were measured at approximately monthly intervals from November 1983 to October 1985. Benzoa]pyrene hydroxylase and ethoxyresorufin O-deethylase activities and cytochromes P450 and b₅ were generally, but not significantly, higher in males than in females. The MFO activity varied seasonally, reaching a maximum during or shortly after spawning. Variation in MFO enzyme activity between sexes was never greater than 2-fold at any time, and within sex, no greater than 6-fold during the seasonal cycle. This variability is less than that caused by exposure to environmentally realistic levels of some pollutants; measurements of MFO activity in this species might therefore be used to indicate environmental contamination.