| An In Vitro Investigation of Pulmonary Alveolar Macrophage Cytotoxicity Introduced by Fibrous and Grainy Mineral Dusts |
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| 作者姓名： | DONG Faqin DENG Jianjun WU Fengchun PU Xiaoyong John HUANG FENG Qiming HE Xiaochun |
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| 作者单位： | [1]Institute of Mineral Materials andApplication, Southwest University of Science and Technology, Mianyang, Sichuan 621002, China [2]Clinical Laboratory, Fourth People＇s Hospital ofMianyang, Mianyang, Sichuan 621000, China [3]Department of Clinical Labor |
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| 基金项目： | 国家自然科学基金;教育部优秀青年教师资助计划 |
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| 摘 要： | In order to study the damage mechanism of mineral dusts on the pulmonary alveolar macrophage （AM）, the changes in their death ratio, malandialdthyde （MDA） content and activities of lactate dehydrogenase （LDH） and superoxide dismutase （SOD） were measured, and the technique of cell culture in vitro was used to investigate the cytotoxicity of six mineral dusts （twelve crystal habits） from twelve mineral deposits. The results show that woUastonite and clinoptilolite have no AM cytotoxicity, while other fibrous and grainy mineral dusts damage pulmonary AM in various degrees. The cytotoxicity of fibrous mineral dusts was greater than that of the grainy ones, and the cytotoxicity of dusts was positively correlated with the active OH- content in dusts, but not necessarily so with its SiO2 content. The high pH values produced by dust was unfavorable for the survival of cells and the dusts with low bio-resistance were safe for cells. The content of variable valence elements in dusts might influence their cytotoxicity and the surface charge of dusts was not a stable factor for their toxicity. It is demonstrated that the shape of mineral dusts was one of the factors affecting cytotoxicity, and that the cytotoxicity of mineral dusts depends mainly on their properties.
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| 关 键 词： | 粒状矿物 纤维矿物 细胞毒性 巨噬细胞 |
| 收稿时间： | 2005-10-26 |
| 修稿时间： | 2006-02-20 |
An In Vitro Investigation of Pulmonary Alveolar Macrophage Cytotoxicity Introduced by Fibrous and Grainy Mineral Dusts |
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| Authors: | DONG;Faqin;DENG;Jianjun;WU;Fengchun;PU;Xiaoyong;John;HUANG;FENG;Qiming;HE;Xiaochun |
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| Abstract: | In order to study the damage mechanism of mineral dusts on the pulmonary alveolar macrophage (AM), the changes in their death ratio, malandialdthyde (MDA) content and activities of lactate dehydrogenase (LDH) and superoxide dismutase (SOD) were measured, and the technique of cell culture in vitro was used to investigate the cytotoxicity of six mineral dusts (twelve crystal habits) from twelve mineral deposits. The results show that wollastonite and clinoptilolite have no AM cytotoxicity, while other fibrous and grainy mineral dusts damage pulmonary AM in various degrees. The cytotoxicity of fibrous mineral dusts was greater than that of the grainy ones, and the cytotoxicity of dusts was positively correlated with the active OH- content in dusts, but not necessarily so with its SiO2 content. The high pH values produced by dust was unfavorable for the survival of cells and the dusts with low bio-resistance were safe for cells. The content of variable valence elements in dusts might influence their cytotoxicity and the surface charge of dusts was not a stable factor for their toxicity. It is demonstrated that the shape of mineral dusts was one of the factors affecting cytotoxicity, and that the cytotoxicity of mineral dusts depends mainly on their properties. |
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| Keywords: | grainy mineral fibrous mineral dust pulmonary alveolar macrophage cytotoxicity |
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