Cloning and developmental expression of kinesin superfamily7 (kif7) in the brackish medaka (Oryzias melastigma), a close relative of the Japanese medaka (Oryzias latipes) |
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| Institution: | 1. Department of Biochemistry, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea;2. Clinical Trial Center for Functional Foods, Chonbuk National University Hospital, Jeonju, Jeonbuk 54907, Republic of Korea;3. Department of Molecular Biology, Chonbuk National University, Jeonju, Jeonbuk 54896, Republic of Korea;4. Oriental Medicine Bio-Technical Fusion Center, Daejeon University Industry Academic Cooperation Foundation, Daejeon 30520, Republic of Korea;5. Department of Pharmacology, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea;6. Department of Surgery, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea;1. Mizuki biotech, Co., Ltd., 1-1 Hyakunenkouen, Kurume, Fukuoka 839-0864, Japan;2. Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan;3. Department of Biological and Chemical Systems Engineering, National Institute of Technology, Kumamoto College, 2627 Hirayama-shinmachi, Yatsushiro, Kumamoto 866-8501, Japan;4. Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan;5. Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Higashi-ku, Tsukide, Kumamoto 862-8502, Japan;1. Department of Biological Sciences, Virginia Tech, 1405 Perry Street, Blacksburg, VA 24061, USA;2. Department of Fish and Wildlife Conservation, Virginia Tech, Blacksburg, VA 24061, USA;3. Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA;4. Virginia Tech Carilion Research Institute, Roanoke, VA 24016, USA;1. Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China;2. Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA |
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| Abstract: | kif7 is a member of the kinesin superfamily members which are molecular motor proteins that move along microtubules in a highly regulated manner through ATP hydrolysis. In this paper, we report on the cloning of the Oryzias melastigma kif7 (omkif7) using primers designed according to the Japanese medaka (Oryzias latipes) database. The cloned omkif7 has an open reading frame of 3762bp and is deduced to encode a polypeptide of 1254 amino acids that possesses the putative ATP-binding and microtubule-binding motifs in its motor domain at the N-terminal region. We characterized the cloned omkif7 by comparison with the zebrafish kif7. Both omkif7 and zebrafish kif7 are shown to be expressed in all embryonic stages and adult tissues examined with higher expression level in the testis and ovary. Whole-mount in situ hybridization revealed that the expression of omkif7 is ubiquitous during the early stages of embryonic development, but became more restrictive and localized to the brain, fin bud and eye at later development. This study suggested that the brackish O.melastigma can serve as a good seawater model organism for developmental studies by utilizing the resources developed from its close relative of the Japanese medaka. |
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