Methods used to study bacterial diversity in the marine environment around Qingdao |
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Authors: | P A W Robertson J MacInnes O A E Sparagano I Purdom Y Li D H Yu Z J Du H S Xu B Austin |
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Institution: | 1. Centre for Marine Biodiversity and Biotechnology CMBB, Department of Biological Sciences, Heriot- Watt University, Riccarton, Edinburgh. EH14 4AS, Scotland, UK 2. The Darwin Unit, Department of Marine Biology, Ocean University of Qingdao, Qingdao 266003, P. R. China |
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Abstract: | Pollution has a considerable effect on biological communities, in terms of size and diversity of the populations. Yet, the
precise consequences of human activity on microbial communities in the marine environment are poorly understood. Therefore,
in an ongoing collaborative research programme between Heriot-Watt University and the Ocean University of Qingdao, bacteria
were isolated in 1999 and 2000 from marine sediment, seawater, seaweed, fish and shellfish, taken from locations in Shandong
Province adjacent to Qingdao. Sampling locations were comprised of industrial and aquacultural sites and a clean, control
site. In order to analyse microbial diversity, a polyphasic approach was adopted for characterisation of these isolates, specifically
through examination of key phenotypic traits, i.e. using Biolog GN MicroPlate™ profiles, bacterial whole cell protein profiles and 16S and 23S rRNA gene sequences. These techniques yielded complex taxonomic
data, which were subjected to statistical and cluster analyses. The application of these methods to studies of microbial communities
is discussed. |
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Keywords: | bacteria marine environment biodiversity Biolog GN |
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