Occurrence of coprostanol, 24-ethylcoprostanol and 5α-stanols in the marine sediments

The present study deals with the elucidation of sterol composition of the marine sediments in Kagoshima Bay. The identification of each sterol was performed by gas-liquid chromatography, Ag⁺ impregnated column chromatography, mass spectrometry, and nuclear magnetic resonance spectrometry. The sediment obtained near the estuary of River Koutsuki contained large portions of 5-stanols such as coprostanol and 24-ethylcoprostanol basides 5-stanols such as cholestanol, 24-methylcholestanol, and 24-ethylcholestanol in the sterol fraction. These 5-stanols in the marine sediment may be derived from the fecal contamination by domestic sewages.