鄱阳湖流域蚌类环境DNA宏条形码引物的筛选验证

环境DNA技术是一种非侵入、高灵敏、高效率且对环境无破坏性,对生物体无损伤的调查工具.为了筛选适合于蚌类环境DNA生物多样性研究的宏条形码引物,本研究通过对鄱阳湖流域24种常见蚌的基因组DNA进行普通扩增和高通量测序筛选了11对引物(设计了9对通用引物及从相关文献中引用了2对引物),结果显示引物cytb和16S rRN...

Universal primers screening and verification for environmental DNA metabarcoding of freshwater mussels in the Lake Poyang Basin

Environmental DNA (eDNA) technology is a non-invasive, highly sensitive, efficient and non-destructive to the environment, no damage to the organism investigation tool. To select suitable metabarcoding primers for freshwater mussel''s environmental DNA biodiversity research, 11 pairs of primers (with 9 pairs of universal primers designed and 2 pairs cited from relevant literature) were screened by general amplification and high-throughput sequencing of genomic DNA of 24 common mussels in the Lake Poyang Basin. The results showed that the primer cyt b and 16S rRNA had good amplification effect and high discrimination. The two pairs of primers were further verified with environmental samples (n=6) and traditional sampling. It was found that 8 mussels species belonging to 6 genera were annotated by 16S rRNA which accounted for 26.69% of the total sequences, and 5 mussels species belonging to 4 genera were annotated by cyt b which accounted for 6.60% of the total sequences. The primer 16S rRNA was more suitable used for metabarcoding primer for the research of environmental DNA biodiversity in freshwater mussels. Environmental DNA technology can be used as an effective supplement to traditional methods in biodiversity monitoring. The reliability and detection rate can be increased by using multiple pairs of primers at the same time.