| 海带杂种优势苗种繁育技术研究 |
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| 引用本文： | 刘涛,王翔宇,崔竞进.海带杂种优势苗种繁育技术研究[J].海洋学报,2005,27(1):145-148. |
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| 作者姓名： | 刘涛 王翔宇 崔竞进 |
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| 作者单位： | 中国海洋大学,海洋生命科学与技术学院,山东,青岛,266003;中国海洋大学,海洋生命科学与技术学院,山东,青岛,266003;中国海洋大学,海洋生命科学与技术学院,山东,青岛,266003 |
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| 基金项目： | 国家“863”计划资助项目(2004AA603210)，山东省教育厅资助项目(J02H53)，中国海洋大学理科基金资助项目. |
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| 摘 要： | 用生物反应技术进行海带配子体克隆的连续培养,将丝状体克隆细胞粉碎后,用两系法培育杂种优势苗种.实验结果表明,雌、雄海带配子体克隆细胞的日增重速率可达14.03%和13.87%,经42d培养增重6.9倍和6.8倍;雌、雄配子体克隆细胞杂交16d,排卵率为100%;培养40d后幼孢子体长度为2~3cm,经海上养殖实验,初步筛选出了一个较好的杂交组合.
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| 关 键 词： | 海带 配子体克隆 两系杂交 杂种优势 |
| 文章编号： | 0253-4193(2005)01-0145-04 |
| 收稿时间： | 2003/7/17 0:00:00 |
| 修稿时间： | 2003/11/7 0:00:00 |
The study of heterosis seedling and seed in Laminaria |
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| LIU Tao,WANG Xiang-yu and CUI Jing-jin.The study of heterosis seedling and seed in Laminaria[J].Acta Oceanologica Sinica (in Chinese),2005,27(1):145-148. |
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| Authors: | LIU Tao WANG Xiang-yu and CUI Jing-jin |
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| Institution: | Ocean University of China, Qingdao 266003, China |
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| Abstract: | By bringing up continously clone gametophyte of Laminaria,make clone cells processing ismade continuously protonema clone gam etophyte is shattered,using twain system crossbreed culling cultivate breed heterosis.The expermient result shows that the increasing-weight rate per day of female and male clone gametophyte of Laminaria is 14.03% and 13.87%,After 42 d cultivating,their weight increasing proportions are 6.9 and 6.8 tmies.The expermient of seedling clone of Lam inaria is a lso done.Under the proportion of 2.1(cells count),female and male clone cultures 16 d,the ovulate rate is 100%.After 60 d cultivated,young sporophyte slength is 2~3 cm,by culturing in the sea,one crossbreed breed is selected. |
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| Keywords: | Laminaria clone gametophyte twain system crossbreed heterosis |
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