| Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean |
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| 作者姓名： | Lin Xuezheng Yang Xiuxi Bian Ji Huang Xiaohang |
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| 作者单位： | Lin Xuezheng,BIAN Ji,Huang Xiaohang(First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China);Yang Xiuxia(Fishery College, Ocean University of China, Qingdao 266003, China) |
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| 基金项目： | This study was supported by 10th five year plan(DY105040203) of China Ocean Mineral Resources R&D Association |
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| 摘 要： | A strain of psychrophilic bacterium, 2-5-10-1, which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern Ocean. The highest lipase secretion of this strain is observed at 5 ℃ and this temperature is also for optimal growth. Tween 80 and olive oil enhance secretion of lipase. The optimal temperature and pH for lipase activity are 35 ℃ and 7.5 ℃ respectively. At 0℃, the lipase still has 37% relative enzyme activity. The lipase shows high thermolability, more than 50% activity lost after incubation at 60 ℃ for 15 min. EDTA has no effect on lipase activity, indicating the lipase activity is independent of divalent cation. In contrast, the lipase activity is inhibited drastically by Cu2 and Zn2 .
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| 关 键 词： | Southern Ocean deep sea psychrophilic bacteria low-temperature lipase |
| 收稿时间： | 2003/3/18 0:00:00 |
| 修稿时间： | 2003/8/12 0:00:00 |
Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean |
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| Lin Xuezheng,Yang Xiuxi,Bian Ji,Huang Xiaohang.Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean[J].Acta Oceanologica Sinica,2003,22(4):643-649. |
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| Authors: | Lin Xuezheng Yang Xiuxi Bian Ji and Huang Xiaohang |
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| Institution: | 1.First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China2.Fishery College, Ocean University of China, Qingdao 266003, China |
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| Abstract: | A strain of psychrophilic bacterium, 2-5-10-1, which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern Ocean. The highest lipase secretion of this strain is observed at 5 ℃ and this temperature is also for optimal growth. Tween 80 and olive oil enhance secretion of lipase. The optimal temperature and pH for lipase activity are 35 ℃ and 7.5 ℃ respectively. At 0℃, the lipase still has 37% relative enzyme activity. The lipase shows high thermolability, more than 50% activity lost after incubation at 60 ℃ for 15 min. EDTA has no effect on lipase activity, indicating the lipase activity is independent of divalent cation. In contrast, the lipase activity is inhibited drastically by Cu2 + and Zn2 +. |
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| Keywords: | Southern Ocean deep sea psychrophilic bacteria low-temperature lipase |
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