A method for quantifying living roots of Spartina (cordgrass) and Juncus (needlerush)

Quantifying living roots in a marsh is a necessary but difficult task in wetland research. The two main difficulties usually encountered are distinguishing living from dead roots and processing a dense mat of fine roots. We found that living roots of salt-marsh plants release much more dissolved organic carbon (DOC) in boiling water than dead roots. Based on the finding, we developed a DOC procedure to quantify living roots of Spartina alterniflora and Juncus roemerianus. The DOC released in boiling water is a function of root activity, and the amount released can be used to calculate the living root biomass of a sample. The amount of living roots determined by the DOC method correlated well with the amount of living roots determined by the manual, sorting method (r² = 0.78, p<0.01). The DOC method is more objective, precise, and much less tedious than the manual sorting method.