Combined effects of heavy metal and polycyclic aromatic hydrocarbon on soil microorganism communities |
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Authors: | Linkui Cao Guoqing Shen Yitong Lu |
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Institution: | (1) Department of Environment and Resource, Shanghai Jiaotong University, Shanghai, 201101, People’s Republic of China |
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Abstract: | Soil contaminated sites contain a variety of pollutants, especially heavy metals and polycyclic aromatic hydrocarbons (PAHs).
Interactions between heavy metals have been relatively well studied, but little is known about interactions between heavy
metals and PAHs. The combined effect of heavy metals and PAHs on soil microorganism was studied in laboratory conditions and
evaluated by random denaturing gradient gel electrophoresis. We extracted DNA directly from contaminated soils and then amplified
the V3 sequences of the 16S rDNA. The results showed that with different culture time, the gene diversity of the single and
combined contaminated soil differed as well. After 15 days of culture, the microorganisms were stimulated and accommodated.
After 45 days of cultivation, the quantities of the soil microorganisms were affected. It is concluded that some of the microorganisms
utilize phenanthrene as important carbon resources. Microorganisms directly isolated from soil could reflect the diversity
of soil microorganism and population distribution conditions. |
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Keywords: | Heavy metal Cd Phenanthrene DGGE Soil microorganisms |
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