Characterization and application of monoclonal antibodies against Shewanella marisflavi, a novel pathogen of Apostichopus japonicus |
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| Authors: | Qiang Li Hongli Jing Hua Li Yinan Wang Dehai Xu |
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| Affiliation: | LI Qiang 1,JING Hongli1,LI Hua 1,**,WANG Yinan 1,XU Dehai2 1 Key Laboratory of Mariculture,Agriculture Ministry of China,Dalian Ocean University,Dalian 116023,China 2 U.S. Department of Agriculture,Agricultural Research Service,Aquatic Animal Health Research Laboratory,Auburn,AL 36832,USA |
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| Abstract: | Shewanella marisflavi strain AP629 was certified as a novel pathogen of the sea cucumber Apostichopus japonicus. In this study, four monoclonal antibodies (MAbs) (3C1, 3D9, 2F2, 2A8) against strain AP629 were developed by immunizing Balb/C mice. 3C1 and 3D9 recognized S. marisflavi only, showing no cross reactivity to other gram-negative bacteria. However, 2F2 and 2A8 showed cross reactivity to all tested bacteria. Indirect immunofluorescence, and immunogold electron microscopy, showed the binding antigens ... |
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| Keywords: | Apostichopus japonicus Shewanella marisflavi monoclonal antibodies |
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