正交实验法优化紫蛇尾皂苷提取工艺研究

以紫蛇尾(Ophiopholis mirabilis)皂苷溶液吸光度为指标,采用单因素实验法考察乙醇体积分数、料液比、提取时间、提取次数、提取温度等因素对紫蛇尾皂苷提取效果的影响,通过正交实验确定最佳工艺条件。结果表明,紫蛇尾皂苷的最佳提取工艺为60℃水浴,料液比1:40(W/V),每次3 h,提取4次。按照最佳工艺条件所得蛇尾皂苷平均产率为1.77‰,表明此最佳工艺可用于紫蛇尾皂苷的制备。

Optimization of the extraction of ophiurasaponin from Ophiopholis mirabilis with the orthogonal design method

With the absorbence of ophiurasaponin solution from Ophiopholis mirabilis as index,the influence of important parameters on the extraction of ophiurasaponin were investigated by the single factor method,such as aqueous ethanol concentration,solid to liquid ratio,steeping duration,times of extraction,extraction temperature;the optimal parameters were determined by orthogonal experiment designing.The results showed that optimal process conditions were water bath temperature of 60℃,solid to liquid ratio of 1:40(W/V),four times extraction with the interval of 3 h.The corresponding average yield of ophiurasaponin is 1.77‰,indicating that the optimal process fits for preparation of ophiurasaponin from O.mirabilis.