青岛市雾天细菌气溶胶群落结构特征及健康风险

本文于2020年7—9月采集雾天和晴天生物气溶胶样品,利用高通量测序和BugBase基因功能预测,评估了雾天细菌气溶胶群落特征及潜在健康风险.结果表明,雾天可操作分类单元(OTUs)、物种多样性及丰富度均低于晴天,组内亲缘复杂度高于晴天.罗尔斯通氏菌、短杆菌、丙酸菌和短波单胞菌是雾天优势细菌属,雾天微生物几乎来源于本土...

Characteristics of Bacterial Aerosol Community Structure and Health Risks in Heavy Fog in Qingdao

The health risk effects of bioaerosols under different weather conditions have received increasing attention.The characteristics of bioaerosols in haze have been extensively studied,but reports on foggy days are relatively rare.The foggy and sunny bioaerosol samples were collected from July to September 2020,and high-throughput sequencing and BugBase gene function prediction were used to evaluate the characteristics of the foggy bacterial community and potential health risks.The results showed that the OTUs,species diversity and richness in foggy days were lower than those in sunny days,and the complexity of kinship within the group was greater than in sunny days.Ralstonia,Brevibacterium,Propionibacterium and Brevundomonas are the dominant bacterias in foggy weather.The microbes in foggy days are almost native,and they are almost the same as the bacterial genera in sunny days,but there are some differences in relative abundance.There was little difference between Gram-negative bacteria(85%)and Gram-positive bacteria(13%)in the two samples,and the relative abundance of potentially pathogenic bacteria was higher in foggy days(73.42%)than that in sunny days(59.95%).Six human-infectious microorganisms were detected in both weathers Corynebacterium,Serratia,Staphylococcus,Neisseria,Acinetobacter and Morganella.The total relative abundance of foggy days is 6.51%,and that of sunny days is 4.42%.Among them,the relative abundance of Corynebacterium,Serratia,and Staphylococcus was not much different under the two weathers.Neisseria has a higher content on sunny days,Acinetobacter and Morganella have higher relative abundance on foggy days.Comprehensive gene prediction results and potential pathogen analysis,foggy weather pathogenic and infectious bacteria are more abundant,and health risks are higher.