Methane-producing bacteria: natural fractionations of the stable carbon isotopes |
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Authors: | Larry M. Games J.M. HayesRobert P. Gunsalus |
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Affiliation: | Departments of Chemistry and Geology, Indiana University, Bloomington, IN 47401, U.S.A.;Department of Microbiology, University of Illinois, Urbana, IL 61801, U.S.A. |
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Abstract: | The fractionation factors () for the reduction of CO2 to CH4 by pure cultures of methane-producing bacteria are, for Methanosarcina barkeri at 40°C, 1.045 ± 0.002; for Methanobacterium strain M.o.H. at 40°C, 1.061 ± 0.002; and, for Methanobacterium thermoautotrophicum at 65°C, 1.025 ± 0.002. These observations suggest that the acetic acid used by acetate dissimilating bacteria, if they play an important role in natural methane production, must have an intramolecular isotopic fractionation () approximating the observed fractionation. |
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