Isolation and purification of Cu-free methanobactin from Methylosinus trichosporium OB3b |
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| Authors: | Marie-Laure Pesch Iso Christl Kurt Barmettler Stephan M Kraemer and Ruben Kretzschmar |
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| Institution: | (1) Department of Environmental Sciences, ETH Zurich, CHN, Institute of Biogeochemistry and Pollutant Dynamics, Universit?tstrasse 16, 8092 Zurich, Switzerland;(2) Department of Environmental Geosciences, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria |
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| Abstract: | Background The isolation of highly pure copper-free methanobactin is a prerequisite for the investigation of the biogeochemical functions
of this chalkophore molecule produced by methane oxidizing bacteria. Here, we report a purification method for methanobactin
from Methylosinus trichosporium OB3b cultures based on reversed-phase HPLC fractionation used in combination with a previously reported resin extraction.
HPLC eluent fractions of the resin extracted product were collected and characterized with UV-vis, FT-IR, and C-1s NEXAFS
spectroscopy, as well as with elemental analysis and ESI-MS. |
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