东海三疣梭子蟹雌体不同生理阶段肝胰腺的生化组成与其组织学结构的关系

通过生化分析和组织切片技术,系统研究了东海三疣梭子蟹第一次卵巢发育过程中肝胰腺指数(HSI)、主要生化成分和组织学结构的变化。结果表明:(1)三疣梭子蟹第一次卵巢发育过程中HSI呈先上升后下降趋势,最大值在Ⅳ期[(7.46±1.23)%],最低值在Ⅵ期[(4.62±0.63)%];(2)三疣梭子蟹第一次卵巢发育过程中,肝胰腺中的生化组成存在显著变化,Ⅰ期肝胰腺中水分含量高达67.91%,其他各期间无显著差异(P>0.05),肝胰腺中脂肪/湿重含量呈先上升后下降趋势,Ⅰ期最低(13.48%),Ⅳ期最高(24.24%);肝胰腺中蛋白/湿重并无显著变化(P>0.05),而总糖/湿重呈显著下降趋势(P<0.05);(3)不同生理阶段雌体肝胰腺组织学存在两个较明显的变化—生殖蜕壳及产卵前后,Ⅰ期肝小管中B细胞占优势(约50%左右),R细胞较细长,肝小管内腔中存在大量食物残渣和脱落的B细胞顶泡等,生殖蜕壳后(Ⅱ期后)的雌体肝胰腺中R细胞数量较多,随着时间的推移R细胞逐渐饱满。产卵后雌体肝胰腺中的细胞间界限变得模糊,部分肝小管的管腔开始自溶,但是此时B,F细胞数量开始增多,这说明随着春季东海海区水温的升高,雌体开始恢复摄食,以保证第二次卵巢发育的顺利进行。

The changes of histology and main biochemical composition in the hepatopancreas at the different physiological stages of Portunus trituberculatus in East China Sea

The study was conducted to investigate the changes in the hepatosomatic index (HSI), the main biochemical compositions and its histological feature of Portunustrituberculatus at the different physiological stages in East Sea by biochemical determination and hist olog ical examination.Furthermore, the relationship between changing histology and main biochemical composition in the hepat opancreas were also discussed in the end of this paper.The results showed that: (1) During the ovarian maturation, the HSI increased to (7.46±1.23)% at stage Ⅳ, then decreased to (4.62±0.63)% at stage Ⅳ; (2) Although the stable protein level (10%~12% wet weight) could be found in wet hepatopancreas during the ovarian development, there were significant differences in the other main biochemical compositions during the course of ovarian maturation.The highest water content of the hepato pancreas could be found at the stage Ⅰ, which reached 67.91%.Then, the total lipid (TL) content based on wet hepat opancreas appeared as the trend of "low—high—low".The peak TL content (24.24% wet weight) was found at stage Ⅳ in wet hepatopancreas, while the lowest TL content was only 13.48% at stage.Moreover, the carbohydrate content of wet hepat opancreas decreased slightly except for stage.(3) The significant changes of hepatopancreatic histology happened in the reproductive molting and spawning stages in female crabs.In early stage Ⅰ (before reproductive molting), B cells dominated in the hepat opancreatic tubules while R cells seemed slender.Meanwhile, the abundant food residue and vacuoles sloughed off by B cells were found in tubule lumens of the hepatopancreas.In stage Ⅱ (after reproductive molting), the numbers of R cells increased significantly, which were filled with lipid droplets with ovarian development.After spawning, the boundary of many hepat opancreatic cells became unclear or even mixed.However, there were more B and F cells on the hepat opancreas than before, which suggested that crabs restart feeding to ensure the second ovarian development as the water temperature increased.